Nematicidal properties and phytochemical screening of tamarindus indica and azadirachta indica extract against burrowing nematode of banana plant in sokoto state muhammad i. Research article preliminary phytochemical screening and in vitro antiinflammatory activity of azadirachta indica leaves by human red blood. Tinospora cordifolia, azadirachta indica and ocimum sanctum leaves extract rc patil, cp kulkarni and ashu pandey abstract in the present study the aqueous, methanol, ethanol and acetone extract of tinospora cordifolia, azadirachta indica and ocimum sanctum leaves extract were screened for the presence of phytochemical. Materials and methods collection of plants the leaves of ocimum sanctum, azadirachta indica and phyllanthus emblica were collected from the local area and shade dried, powdered. Antimicrobial activity, azadirachta indica, minimum inhibitory concentration, oral strains, phytochemistry. Neem azadirachta indica is a member of the meliaceae family and its role as healthpromoting effect is attributed because it is rich source of antioxidant.
Algicidal effects of aqueous leaf extracts of neem. Phytochemical screening and antimicrobial activity. Phytochemical, screening, azadirachta indica, nigeria. Preliminary phytochemical screening and in vitro anti. In the present study, we investigated the potential of aqueous crude extract of azadirachta indica to inhibit the growth of scenedesmus quadricauda. Ferric chloride 1% negative flavonoids positive anthraquinone negative cardiotonics glycosides negative alkaloids negative tannins. Pdf phytochemical, antimicrobial, cytotoxic, analgesic. The present study was undertaken to prepare crude extracts of neem leaves with different polarity organic solvents by using a maceration method and assessing phytochemical screening, the total phenol content and antioxidant activity by the spectroscopic method. The dataset on phytochemical screening of three extracts of azadirachta indica and cymbopogon citratus is presented in table 1.
The present study includes the phytochemical detection and antimicrobial activity of the leaves of azadirachta indica, a. Balarabe science laboratory technology, ramat polytechnic maiduguri borno state nigeria abstract studies were conducted to determine the phytochemicals present in neem azadirachtaindica leaves. Phytochemical extraction and antimicrobial studies on crude leaf. Study of phytochemical screening of neem azadirachta indica. The phytochemical screening of the three extracts of azadirachta indica exhibited the presence of important secondary metabolites such as flavonoids, terpenoids, steroids and tannins. Pdf the phytochemical screening of neem leaf extract was conducted to assess the nature and concentrations of phytochemicals useful in. Micromorphological and preliminary phytochemical studies of azadirachta indica and melia azedarach samineh jafari1,2. Neem azadirachta indica is widely used in the ayurvedic medicine system for treating malaria and fever. The fact that many traditional remedies are of therapeutic value. Pdf preliminary phytochemical analysis of hibiscus. The fact that many traditional remedies are of therapeutic value is no longer in doubt. Micromorphological and preliminary phytochemical studies of azadirachta indica and melia azedarach samineh jafari1,2, soodabeh saeidnia3, mohammad reza shams ardekani 1,4, abbas hadjiakhoondi1,3, mahnaz khanavi1,4, 1department of pharmacognosy, faculty of pharmacy, tehran university of medical sciences, tehran 141556451, iran. Nematicidal properties and phytochemical screening of. Phytochemical screening and antibacterial activity of extracts of azadirachta indica a.
Phytochemical screening of the leaf extract of leaves both fresh and shade dried indicated the presence of flavonoids, terpenoids. Phytochemical screening, quantitative estimates of bioactive compounds in spondias mombin and azadirachta indica. Phytochemical screening and antimicrobial activity evaluation. In this study the ethanol extract, nhexane extract and chloroform extract of azadirachta indica were first evaluated for phytochemical study. Phytochemical screening and effects of methanolicextract. Antifungal and phytochemical properties of tinospora. Phytochemical screening, quantitative estimates of bioactive compounds in spondias mombin and azadirachta indica ogbonna o. Department of biochemistry, ladoke akintola university of technology, ogbomoso, nigeria.
Phytochemical screening and antimicrobial activity of. Quantitative phytochemical screenings were done to determine the amounts of phytochemical that are present in the crude extracts. It is hoped that these active constituents will provide useful information for discovering new compounds. Extraction of phytochemicals and study of antimicrobial properties of azadirachta indica neem alpana deshpande a1 and bhagyashree deshpande b adepartment of home science, dr. Phytochemical screening was carried out to identify major biologically active phytoconstituents. Analysis of phytochemical variability in neem formulations. The aim of this study was to evaluate the antioxidant activity, screening the phytogenic chemical compounds, and to assess the alkaloids present in the e. Micromorphological and preliminary phytochemical studies. Phytochemical profiling of phytoconstituents of grape. Biological activities of different neem leaf crude. This study includes the preliminary phytochemical screening and quantitative analysis of secondary metabolites of leaves and stems extract of mentha arvensis and azadirachta indica.
Qualitative phytochemical analysis demonstrated the presence of tannins and saponins in all plants tested. Phytochemical analysis of plant extracts table 1 presents the result of the phytochemical screening of the stem bark of azadirachta indica. The result showed that the extract of azardirachta indica neem possessed active principles which include. Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of a. Phytochemical extraction and antimicrobial properties of. Phytochemical screening of the extract revealed the presence of groups of bioactive compounds that are capable of inhibiting microalgal growth. Phytochemical profiling of phytoconstituents of grape, jatropha curcas and neem azadirachta indica extracts olabinri b.
The analysis was carried out utilizing standard methods and protocols. Azadirachta indica, li, bacillus subtilis and staphylococcus aureus, antibacterial activity, cup plate method introduction natural herbs and their varied extracts have been used globally in therapeutic since antiquity summar 9. Phytochemical screening was carried out on extract by. Escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and salmonella typhi by disc. The next step involves a retrieval of ethnobotanical information to discern possible bioactive molecules.
Phytochemical screening of the leaf extract of leaves both fresh and shade dried indicated the presence of flavonoids, terpenoids, tannins, alkaloids, steroids and glycosides. Phytochemical analysis of methanolic leaf extracts of azadirachta indica has shown the presence of biological compounds like, alkaloids, flavonoids, saponins, etc which are then compared. The fingerprint profiles of methanol extract and ethyl acetate sub fraction of azadirachta indica leaves were conducted using preliminary phytochemical screening and high performance thin layer chromatography hptlc analysis. Antibacterial activities of azadirachta indica against. Preliminary phytochemical evaluation and hptlc fingerprinting. The antibacterial activity of aqueous pulp extract of this plantwas carried out against four bacteria. The genotoxic effect of the plant is due to the most active principle azadirachtin khan and aswathy, 2003. Pdf micromorphological and preliminary phytochemical. The present study was aimed to investigate the phytochemical screening and antimicrobial assessment of adhatoda vasica, azadirachta indica and datura stramonium leaf extract.
The leaf extracts of azadirachta indica and spondias mombin were prepared using different solvents like methanol, chloroform and petroleum ether and were screened for their pesticidal properties. Azadirachta excelsa leaves were collected from forest research institute malaysia frim in kepong, selangor, malaysia. Crude leaf extracts of azadirachta indica neem was subjected to in vitro phytochemical screening and antimicrobial assays against some selected microbial. The phytochemical screening of the aqueous and ethanolic extracts of. The study was carried out to evaluate the pesticidal properties of azadirachta indica and spondias mombin leaves against the activities of phytolyma lata. Moreover, we investigated the biological activity of the potent crude extracts against s. The phytochemical analysis of extracts were performed, and antimicrobial activity of aqueous and alcoholic extracts. Secundary metabolite results saponins positive tannins gelatin 2. Antioxidant activity was analyzed by using 2,2diphenyl1picrylhydrazylhydrate assay.
Phytochemical screening, quantitative estimates of. Preliminary phytochemical screening was performed using various biochemical tests. Jun 03, 2016 phytochemical analysis and antimicrobial activity of methanolic, ethanolic and acetonic extracts of stem bark and leaf of neem plant azadirachta indica effiong edet bassey 1, gwana adamu mohammed 2, halima mohammed bala 3, umeh sophina ogonna 1, bagudu buhari yawuri 4, okoli c. The phytochemical screening indicates the presence of alkaloids, flavonoids, saponins, steroids, tannins and phenolic compounds.
Ftir spectra and data of different crude extracts of each plant are presented in fig. The present investigation was undertaken to screen the phytochemical analysis and antioxidant activity of azadirachta indica plant extracts. S2, ismaila a3, musa a2 1department of biological sciences, usmanu danfodiyo university sokoto, nigeria. Alkaloids, saponins, flavonoids, phenols and tannin which were present in the crude extracts. Phytochemical screening preliminary qualitative phytochemical screening was carried out with the following methods shanmugam et al. Phytochemical characterization, in vitro antibacterial activity, in vivo acute toxicity studies of the seed oil of azadirachta indica neem oil in wistar rats volume 5 issue 1 2019 wirsiy leonel ngum,1 gonsu hortense,2 ngameni barthelemy,3 tembe estella, 1 fokunang charles ntungwen1 1department of pharmacotoxicology and pharmacokinetics. The phytochemical investigation showed positive result for carbohydrates, reducing sugars, protein, amino acids, alkaloids, flavonides in case of aqueous extract and ethanol extracts. Phytochemical screening of the extracts table 1 shows the phytochemical screening of the extract of the a. Azadirachta indica, neem, active principles, limonoids, phytochemical variability, hplc. Phytochemical analysis of saraca asoca bark and azadirachta. Research article phytochemical analysis of some traditional. Phytochemical analysis gave positive results for steroids, phenolic compounds, alkaloids, glycosides, terpenoids, flavonoids and tannins. The aqueous pulp extract of this plant was obtained using hot water extraction method.
Oct 14, 2019 quantitative phytochemical screenings were done to determine the amounts of phytochemical that are present in the crude extracts. Pdf preliminary phytochemical analysis of hibiscus rosa. Micromorphological and preliminary phytochemical studies of. The extracts showed potential antimicrobial activities against. Phytochemical screening, cytotoxic, genotoxic and mutagenic effects of the aqueous extract of azadirachta indica leaves article pdf available april 2017 with 507 reads how we measure reads. Phytochemical screening and antimicrobial activity evaluation of. Preliminary phytochemical screening, quantitative analysis. Phytochemical analysis and antibacterial evaluation of. Phytochemical screening and antimicrobial activity of fresh. Juss neem on some clinical bacterial isolates 3 rd world congress on medicinal plants and natural products research october 0203, 2017 kuala lumpur, malaysia. Phy to chemical screening of azadirachta indica neem. In the present research, a clear systematic investigation of phytochemical screening and antibacterial activity of herbal plants such as azadirachta indica and plectranthus amboinicus.
Azadirachta indica melia azedarach are used to and treat a broad range of ailments zargari, 1990. Phytochemical screening and antidiabetic effects of methanolic extract of azadirachtaindica leaf was investigated in alloxan induced diabetic rats. The phytochemical screening of the aqueous, ethanolic. Neem oil is an indigenous product and a practical solution to curtail mosquito nuisance mishra et al. Phytochemical screening and antimicrobial assessment of.
Pdf phytochemical and biochemical characterizations from leaf. Pdf phytochemical analysis of saraca asoca bark and. College, bhilai, chhattisgarh, india bdepartment of biotechnology and microbiology, bhilai mahila mahavidyalaya, bhilai, chhattisgarh, india abstract. Phytochemical screening and antimicrobial activity evaluation of aqueous and ethanolic extracts of the leaf of azadirachta indica juss neem on some microorganisms itelima et al.
It was concluded that the extract contains pharmacologically active constituents. Azadirachta indica is the medicinally potential plant which is used to cure various skin diseases such as acne, psoriasis and used for the treatment of diabetes and athletes. Phytochemical, antimicrobial, cytotoxic, analgesic and. To evaluate the scientific basis for the use of azadirachta indica, both aqueous and ethanolic extracts of the dried leaves of the plant were subjected to phytochemical screening and determination of antimicrobial activity on six different species of bacteria and a fungus.
The aim of the present study was to investigate the phytochemical screening and in vitro antioxidant activity of aqueous and ethanolic leaf extract of hibiscus rosasinensis and azadirachta indica. Pdf preliminary phytochemical screening and quantitative. Phytochemical, antimicrobial, cytotoxic, analgesic and anti. Phy to chemical screening of azadirachta indica neem meliceace. Phytochemical screening and antimicrobial activity of some medicinal plants against multidrug resistant bacteria from clinical isolates. Objectives of this study is to determine the phytochemical screening of the extract of neem azadirachta indica seed oil,as well as determine the antibacterial effect of extract of neem seed oil on four bacterial isolates. Phytochemical screening and antibacterial activity of. The acidic nature and the antibacterial activity of tamarindus indica obtained from the result suggests its usefulness in preservation of food items against contamination by e. Quantitative phytochemical analysis and antifungal. It indicated that phytochemical screening of the ethanolic extract of a. The phytochemical screening on the qualitative analysis shows that the leaves of azadirachtaindica are rich in flavonoids, tannins and saponins. The phytochemical analysis and antibacterial activity of aqueous pulp extract of tamarindus indica were studied. Ferric chloride 1% negative flavonoids positive anthraquinone negative cardiotonics glycosides negative alkaloids negative tannins, flavonoid, glycoside cardiotonics, saponins. It has been widely used in chinese, ayurvedic, and unani medicines worldwide especially in indian subcontinent in the treatment and prevention of various diseases.
Mar 01, 2016 neem azadirachta indica is a member of the meliaceae family and its role as healthpromoting effect is attributed because it is rich source of antioxidant. It is commonly used in shampoos for treating dandruff and in a soaps or creams for skin conditions. Phytochemical characterization, in vitro antibacterial. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of anabasis aphylla l. Phytochemical screening of the plant from azadirachta indica. Phytochemical analysis of methanolic leaf extracts of azadirachta indica has shown the presence of biological compounds like, alkaloids. Pdf phytochemical screening, cytotoxic, genotoxic and. Therapeutics role of azadirachta indica neem and their. The extracts of the leaves and the stem bark were prepared and screened for the presence of different phytochemicals. Phytochemical screening and antimicrobial activity of some. Pdf phytochemical, antimicrobial, cytotoxic, analgesic and.
About 1ml of leaf extract and 1ml of wagners reagent dilute iodine solution are added and mixed. Phytochemical screening, quantitative estimates of bioactive. Phytochemical, screening, azadirachta indica, nigeria introduction neem or nim which belongs to the family meliaceae, originated from south asia, but grows widely in india, pakistan and other tropical and subtropical parts of the world bokhari and aslam, 1985. The present investigation was undertaken to screen the phytochemical analysis and antioxidant activity of azadirachta. Analysis of phytochemical variability in neem formulations s gunasekaran 1 and b anita 2 1periyar univeristy, salem 636 011, tamil nadu. Phytochemical analysis and antimicrobial activity of.
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